Indaziflam is an alkylazine herbicide that controls winter and summer annual weeds in bermudagrass ( Cynodon sp.) turf by inhibiting cellulose biosynthesis. Research was conducted in Tennessee and Texas during 2010 and 2011 to evaluate the effects of indaziflam applications on overseeded perennial ryegrass ( Lolium perenne ) establishment and summer annual weed control. In Texas, perennial ryegrass cover on plots treated with indaziflam at 0.75 and 1.0 oz/acre measured 37% to 48% compared with 88% for the untreated control 257 days after initial treatment (DAIT). Perennial ryegrass cover following applications of indaziflam at 0.5 oz/acre measured 84% 257 DAIT and did not differ from the untreated control on any evaluation date. Inconsistent responses in crabgrass ( Digitaria sp.) control with indaziflam at 0.5 oz/acre were observed in Tennessee and Texas. However, control was similar to the 0.75-oz/acre rate and prodiamine at 7.8 oz/acre at each location. A September application of indaziflam at 0.75 oz/acre followed by a sequential treatment at 0.5 oz/acre in March of the following year provided >90% control by June 2011. Indaziflam application regimes of this nature would allow for successful fall overseeding of perennial ryegrass every two years and control winter annual weed species such as annual bluegrass ( Poa annua ).