Lisianthus growers look for methods to minimise the emission of nutrients, crop protection chemicals and CO2. In 2014 and 2015, nine crops with Lisianthus have been tested at the Delphy Improvement Centre. This report describes the four trials that have been carried out in the extended research in 2016. With this extension, a distinction was made between different substrates and intensities of assimilation lighting. In addition to knowledge about light use efficiency, water use, heat use, substrate differences and growth development, these extra crop cycles have brought to light that growing Lisianthus on substrate gives a less resilient plant against soil fungi than was experienced during the first crop cycles.