The aim of the experiments was to determine the effect of sowing density and nitrogen fertilisation on the cannabidiol (CBD) and Δ9-tetrahydrocannabinol (Δ9-THC) yields in selected cultivars of fibre hemp. A three-factor field experiment was conducted in 2014-2016. Factor A: cultivar (Futura 75, KC Dora and Tygra), factor B: sowing density (60 and 180 seeds/m2) and factor C: nitrogen fertilisation (0, 30, 60 and 90 kg/ha). The CBD yield ranged from 10.52 g/m2 in cv. Tygra to 10.99 g/m2 in cv. KC Dora. Among the examined cultivars, the highest yield of Δ9-THC in cv. KC Dora was observed. Sowing density did not modify the CBD yield, but increasing the density from 60 to 180 seeds/m2 caused an increase in the yield of Δ9-THC in cvs. KC Dora and Tygra. Nitrogen fertilisation significantly influenced the yields of CBD and Δ9-THC. In both cases, yield increased until the rate of 60 kg N/ha.