Abstract In this study I provide a phylogenetic hypothesis for didelphid Marsupials including a suite of 114 postcranial characters. The postcranial evidence was cladistically analyzed separately and concatenated with a nonmolecular data set previously published (71 cranio-dental-external characters). A combined analysis was done including published IRBP (interphotoreceptor retinoid binding protein, 1158 bp), DMP-1 (dentin matrix protein 1, 1176 bp), and RAG-1 (recombinase-activating gene, 2790 bp) sequences to the nonmolecular data set. In order to compare and evaluate the influence of the inclusion of postcranial morphology to previous hypotheses, the taxon sampling of didelphine ingroup was almost similar to the one used in recent series of papers on didelphid phylogeny. The postcranial information includes 48 characters from the axial skeleton, 37 from the anterior limb, and 29 from the posterior limb. I present anatomical descriptions for each postcranial character, adding details of different condit...