Interests, personality, and values figure prominently in work motivation, yet little research has examined the combined influence of these factors on vocational behavior. The present study therefore examined relationships among these variables in a sample of 282 medical students (169 women, 113 men) who responded to the Strong Interest Inventory, NEO Personality Inventory—Revised, and the Physician Values in Practice Scale. Supporting prior research, results indicated significant relationships between openness and artistic interests and between extraversion and enterprising interests, social interests, and management work values. Regression analyses indicated that personality and vocational interests predicted between 2% and 14% of the variance in each of six work values measured. Personality traits and vocational interests appear to play a meaningful, albeit limited, role in determining work values.