Views provided by UsageCountsHistone H3.3 regulates mitotic progression in mouse embryonic fibroblasts
Copyright policy ) Ors, Aysegul; Papin, Christophe; Favier, Bertrand; Roulland, Yohan; Dalkara, Defne; Ozturk, Mehmet; Hamiche, Ali; +2 Authors
Ors, Aysegul; Papin, Christophe; Favier, Bertrand; Roulland, Yohan; Dalkara, Defne; Ozturk, Mehmet; Hamiche, Ali; Dimitrov, Stefan; Padmanabhan, Kiran;
Histone H3.3 regulates mitotic progression in mouse embryonic fibroblasts
H3.3 is a histone variant that marks transcription start sites as well as telomeres and heterochromatic sites on the genome. The presence of H3.3 is thought to positively correlate with the transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B, combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in the cells, our transcriptomic analyses show very little impact on global gene expression or on the localization of histone variant H2A.Z. Instead, fibroblasts displayed slower cell growth and an increase in cell death, coincident with large-scale chromosome misalignment in mitosis and large polylobed or micronuclei in interphase cells. Thus, we conclude that H3.3 may have an important under-explored additional role in chromosome segregation, nuclear structure, and the maintenance of genome integrity.
Turkey, Turkey, Canada, France
Mouse embryonic fibroblasts, animal cell, Mitotic progression, fibroblast, fluorescence activated cell sorting, Histones, transcriptomics, Mice, Transcription start site, histone H3, Chromosome Segregation, Cells, Cultured, Mammals, Genome, Cell Death, [SDV.BDD.EO] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, protein function, unclassified drug, Nucleic acids, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], immunofluorescence test, Short hairpin RNA, Transcription, Cell death, 570, regulatory mechanism, Cells, Mitosis, RNA sequence, histone, chromatin immunoprecipitation, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, H3.3, histone H3.3, Chromosomes, Article, reverse transcription polymerase chain reaction, transcription initiation site, micronucleus, Animals, controlled study, Nuclear structure, mouse, Cell Proliferation, Cell Nucleus, Molecular Biology/Genomics [q-bio.GN], nonhuman, Fibroblasts, molecular dynamics, Mice, Mutant Strains, Chromosome segregation, Mice, Inbred C57BL, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Genes, genetic variation, Cell culture, Gene expression, RNA-seq, Cytology
Mouse embryonic fibroblasts, animal cell, Mitotic progression, fibroblast, fluorescence activated cell sorting, Histones, transcriptomics, Mice, Transcription start site, histone H3, Chromosome Segregation, Cells, Cultured, Mammals, Genome, Cell Death, [SDV.BDD.EO] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, protein function, unclassified drug, Nucleic acids, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], immunofluorescence test, Short hairpin RNA, Transcription, Cell death, 570, regulatory mechanism, Cells, Mitosis, RNA sequence, histone, chromatin immunoprecipitation, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, H3.3, histone H3.3, Chromosomes, Article, reverse transcription polymerase chain reaction, transcription initiation site, micronucleus, Animals, controlled study, Nuclear structure, mouse, Cell Proliferation, Cell Nucleus, Molecular Biology/Genomics [q-bio.GN], nonhuman, Fibroblasts, molecular dynamics, Mice, Mutant Strains, Chromosome segregation, Mice, Inbred C57BL, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Genes, genetic variation, Cell culture, Gene expression, RNA-seq, Cytology
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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