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Expression Strategy of Aedes albopictus Densovirus

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 2013 Canada, France English Publisher:American Society for MicrobiologyJournal:Journal of Virology, volume 87, pages 9,928-9,932 (issn: 0022-538X, eissn: 1098-5514,

Authors: Pham, Hanh T; Jousset, Françoise-Xavière; Perreault, Jonathan; Shike, Hiroko; Szelei, Jozsef; Bergoin, Max; Tijssen, Peter;

doi: 10.1128/jvi.01259-13

pmid: 23804640

pmc: PMC3754130

Expression Strategy of Aedes albopictus Densovirus

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Abstract

ABSTRACT The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.

Countries

Canada, France

Related Organizations

Département Sciences sociales, agriculture et alimentation, espace et environnement
France
Pennsylvania State University
United States
French National Centre for Scientific Research
France
University of Montpellier
France
Penn State Milton S. Hershey Medical Center
United States

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Keywords

570, [SDV]Life Sciences [q-bio], Molecular Sequence Data, 590, Gene Expression, -, Genome, Viral, Viral Nonstructural Proteins, Cell Line, Viral Proteins, Aedes, Animals, Densovirus, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, RNA, Messenger, Promoter Regions, Genetic, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Base Sequence, [SDV] Life Sciences [q-bio], [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, RNA, Viral, Transcription Initiation Site, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology

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