Purpose – The purpose of this paper is to study the cultural integration, the role of national libraries in regional and international cooperation, Economic Cooperation Organization (ECO) national libraries’ barriers of cooperation and drivers of their cooperation and to propose a framework (conceptual and operational) for their cooperation. Design/methodology/approach – Data collection tool of this survey was a researcher-made questionnaire, sent to 30 individuals and experts involved in cooperation of ECO National Libraries from eight institutions. The reliability of the questionnaire was calculated using Cronbach’s alpha. It was 0.91. In this study, for awareness of activities between ECO national libraries and extraction of drivers, some documents were studied, and preliminary data were collected by informal interviews with meeting stakeholders and participants. Findings – The lack of stability in management positions was one of the major barriers in cooperation of the ECO national libraries. Other major obstacles in this context were lack of continuous communication, lack of common protocols and standards, lack of planning, lack of a central coordinating organization, bureaucracy, etc. Geographical proximity, common history and heritage, common resources, Islamic and cultural factors, importance of information and knowledge, requirements of the international system and political and security requirements were considered as drivers. An information centre was proposed for their active cooperation. Iran is better to host the information centre. Originality/value – National libraries as the scientific and cultural memory of nations and heirs of written heritage should cooperate and share their valuable scientific and cultural heritage to the future generations.