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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Acta Crystallographi...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Acta Crystallographica Section D Structural Biology
Article . 2017 . Peer-reviewed
License: IUCr Copyright and Licensing Policy
Data sources: Crossref
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
HAL AMU
Article . 2017
Data sources: HAL AMU
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
HAL INRAE
Article . 2017
Data sources: HAL INRAE
Acta Crystallographica Section D Structural Biology
Article . 2017
Data sources: Europe PubMed Central
https://dx.doi.org/10.1107/s20...
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Toscana virusnucleoprotein oligomer organization observed in solution

descriptionPublicationkeyboard_double_arrow_right Article 28 Jul 2017 France Publisher:International Union of Crystallography (IUCr)Journal:Acta Crystallographica Section D Structural Biology, volume 73, pages 650-659 (issn: 2059-7983, Copyright policy )
Authors: Baklouti, Amal; Goulet, Adeline; Lichiere, Julie; Canard, Bruno; Charrel, Remi; Ferron, Francois; Coutard, Bruno; +1 Authors

doi: 10.1107/s2059798317008774

pmid: 28777080

Toscana virusnucleoprotein oligomer organization observed in solution

Abstract

Toscana virus(TOSV) is an arthropod-borne virus belonging to thePhlebovirusgenus within theBunyaviridaefamily. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.

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Keywords

negative-stain TEM, Models, Molecular, nucleoprotein oligomers, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Protein Conformation, Bunyaviridae Infections, Crystallography, X-Ray, ribonucleoprotein, X-Ray Diffraction, Scattering, Small Angle, phlebovirus, oligomers, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, Toscana virus, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], negative-sense single-stranded RNA virus, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, nucleoproteins, Sandfly fever Naples virus, SAXS, Nucleocapsid Proteins, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Solutions, Nucleoproteins, [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA, Viral, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Protein Multimerization

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
10
Top 10%
Average
Average
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