Towards a Systematic Validation of References in Real-Time RT-PCR

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2008 English Publisher:Oxford University Press (OUP)Journal:The Plant Cell, volume 20, pages 1,734-1,735 (issn: 1040-4651, eissn: 1532-298X,

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Authors: Laurent, Gutierrez; Mélanie, Mauriat; Jérôme, Pelloux; Catherine, Bellini; Olivier, Van Wuytswinkel;

doi: 10.1105/tpc.108.059774

pmid: 18664615

pmc: PMC2518241

Towards a Systematic Validation of References in Real-Time RT-PCR

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Abstract

Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon et

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Keywords

Reverse Transcriptase Polymerase Chain Reaction, Plant Cells, Reproducibility of Results, Plants

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