Hypothesizing that fish predation, active shore avoidance and outlet stream avoidance may be separately affecting horizontal zooplankton distribution, the effects of fish presence, sampling location (midlake, outlet and non-outlet shore) and time (day or night) on zooplankton abundance and body size were tested. Statistically significant horizontal zooplankton abundance gradients occurred in both fish-present and fish-absent lakes. Fish may strengthen zooplankton spatial patterns common to both fish-present and fish-absent lakes, as abundance differences among locations were often greater in fish-present systems compared to fish-absent systems. Horizontal zooplankton abundance gradients differed through a diel cycle, but were species specific with some species exhibit- ing gradients only during the day, while others exhibit gradients only during the night. Avoidance of the outlet over and above active shore avoidance appeared to take place in Daphnia sp. Other taxa provided equivocal support of active outlet avoidance with most showing no significant difference between shore and outlet abundance (seven of nine), one taxa showing a significant decrease and one a significant increase in outlet compared to shore abundance. No gradients in zooplankton body size were found.