The genus Myrsine L., has been revised for Australia. The broader circumscription of the genus including Rapanea Aubl., has been followed. Twenty species are described, including twelve new species: Myrsine arenaria Jackes, M. angusta Jackes, M. elata Jackes, M. ireneae Jackes, M. kimberleyensis Jackes, M. maculata Jackes, M. oreophila Jackes, M. pedicellata Jackes, M. richmondensis Jackes, M. rubiginosa Jackes, M. serpenticola Jackes, M. smithii Jackes. One new combination is made, Myrsine howittiana (F. Muell. ex Mez) Jackes. Four species M. howittiana (F. Muell. ex Mez) Jackes, M. porosa F. Muell., M. variabilis R.Br., and M. subsessilis F. Muell., are lectotypified. New subspecies described are M. ireneae subsp. curvata and M. subsessilis subsp. cryptostemon. All appropriate combinations have been made for Australian mainland species as well as for species occurring on Lord Howe and Norfolk Islands. A key to the species is provided. The main character states are discussed, particularly the nature of the trichomes, the glands or secretory structures, and aspects of the inflorescence and the flowers.