Natural variation in longevity among populations of the same species, and between species and genera was investigated to inform seed-collection strategies. Seed longevity for 30 wild Australian populations was measured with a controlled ageing test. The populations were represented by eight species from three genera, namely Minuria (Asteraceae), Wahlenbergia (Campanulaceae) and Plantago (Plantaginaceae), each collected from up to eight different locations. Seed-survival curves were fitted by using the equation v = Ki + p/s, which allowed comparison of the initial population viability (Ki), the population distribution of seed life spans (s), and mean seed longevity (P50, calculated as Ki × s). At a genus level, the average P50 indicated that M. integerrima (DC) Benth. is the longest-lived, Wahlenbergia is intermediate and Plantago is the shortest-lived. However, there was also variation in P50 values among populations of most species. Some species had the same s value for all populations, e.g. all eight populations of W. communis Carolin had the same s value, with the differences in Ki causing the variation in P50. This consistency in s existed even though seedlots were collected from diverse locations, with mean annual rainfall ranging from 180 to 840 mm. In comparison, for the six seedlots of W. gracilis (G.Forst.) A.DC., a large difference in s as well as Ki led to the variability in P50, with some indication of a possible correlation between annual rainfall and P50 or s in some species. A relationship between variation in s and the breeding system is proposed for Wahlenbergia. The data show that it can be risky to expect accurate prediction of seed longevity for a wild species on the basis of survival data from a single collection.