Inductively coupled plasma atomic emission spectrometry (ICP-AES) was used for the determination of the elements present in pumpkin seed oil and pumpkin seeds. After extraction of the oil from pumpkin seeds a residue was obtained. For the determination of elements three types of samples were used: oil, seeds and the residue (after oil extraction). Three different digestion types were applied: open vessel and closed vessel digestion in a steel bomb as well as microwave digestion in a closed system. For most elements of interest the measured concentration differs depending on the digestion method used. After microwave digestion a good reproducibility for ICP-AES measurements for all elements was found. Determination of Zn by ICP-AES is possible both after open and closed vessel digestion (steel bomb or microwave digestion). No loss of volatile compounds in pumpkin oil during preparation or microwave digestion prior to ICP-AES analysis could be observed. In general, the recoveries for all elements in pumpkin seed oils and seeds were > 95%, only for S they were 0.8 mg/g for Al, Cr, P, and S in pumpkin oil samples.