Frequent Occurrence of Transgene Deletion in Transgenic Plants
Copyright policy )Frequent Occurrence of Transgene Deletion in Transgenic Plants
The status of the transgene in tobacco plants transformed by Agrobacterium was analyzed with PCR. Twelve percent of the transgenic plants with the nptII gene showed different levels of transgene deletion, which was also found in transgenic watermelon (10-30%) and carrot (40-60%). It appeared that the percentage of transgenic plants carrying deleted transgenes depended on both the transgene and the plant. It is suggested that the transgene should be inserted between a right border and a selection marker to reduce the number of transgenic plants containing deleted transgenes after selection.
- Korean Association Of Science and Technology Studies Korea (Republic of)
- Korea Research Institute of Bioscience and Biotechnology Korea (Republic of)
- National Research Council of Science and Technology Korea (Republic of)
- Korea Advanced Institute of Science and Technology Korea (Republic of)
DNA, Bacterial, Nicotiana, DNA, Complementary, DNA, Plant, Glucose-1-Phosphate Adenylyltransferase, Plants, Genetically Modified, Nucleotidyltransferases, Plants, Toxic, Agrobacterium tumefaciens, Gene Deletion, Solanum tuberosum
DNA, Bacterial, Nicotiana, DNA, Complementary, DNA, Plant, Glucose-1-Phosphate Adenylyltransferase, Plants, Genetically Modified, Nucleotidyltransferases, Plants, Toxic, Agrobacterium tumefaciens, Gene Deletion, Solanum tuberosum
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