Abstract Macronutrient contents of 94 genotypes from the Colombian Central Collection (CCC) and 13 genotypes from the Native Collection (NC) of diploid potatoes (Solanum tuberosum Group Phureja) were evaluated, considering six genotypes, four diploid and two tetraploid potato commercial cultivars, as control. Dry matter, protein, soluble dietary fibre (SDF) and insoluble dietary fibre (IDF), fat, and ash contents were determined in entire boiled tubers. Macronutrient contents varied widely among the 113 genotypes. Protein contents found in CCC and NC (1.8–17.2 g/100 g dry weight, DW) were greater than those in commercial cultivars (3.1–9.5 g/100 g DW). Total dietary fibre (TDF), SDF, and IDF were greater in CCC and NC (9.4–27.1 g/100 g DW, 1.6–6.6 g/100 g DW, and 6.4–20.9 g/100 g DW, respectively) than in the commercial cultivars (9.0–15.3 g/100 g DW, 2.7–4.4 g/100 g DW, and 6.4–12.3 g/100 g DW, respectively). Several genotypes from CCC and NC are good candidates as parental materials for developing new cultivars, with the highest protein content in CCC-76 (17.2 ± 0.3 g/100 g DW) and NC-8 (16.7 ± 0.7 g/100 g DW) and the highest TDF in CCC-110 (27.1 ± 4.0 g/100 g DW).