Within ‘green economy’ approach, growing area of research in the development of green technologies is devoted to designing new, more environmentally friendly solvents. As a promising alternative to traditional organic solvents deep eutectic solvents (DESs) have been dramatically expanding in popularity last few years as a new generation of designer solvents with possible applications in various industrial fields. Herein, we will present recent progress of our multidisciplinary studies on DESs application in biotechnology. Within this study a number of DESs based on renewable sources (e.g. choline chloride, sugar, amino acids and carboxylic acid) were prepared, and screened for their applicability as green solvents in extraction of phenolic compounds from grape skin and lipasecatalyzed synthesis of short chain ester. In order to fully define DESs as environmentally benign, and thus the processes involving them, toxicity profiles of these solvents were also studied. The data obtained would serve to fill the existing gaps in the knowledge regarding DESs application in the extraction of various natural products from plant material as well as in biocatalytic processes. Data present here are our proof-of-concept for possible implementation of DESs into truly green industrial processes.