Abstract Sap is transported under tension (i.e. negative pressure) in trees, according to the tension-cohesion theory. Since water is physically instable under negative pressure, a risk of cavitation is possible. Techniques have been developed during the past two decennia to study cavitation in trees. Trees appear remarkably immune to cavitation events. Cavities form only when extreme water stresses occur or when sap freezes. Nucleation is heterogeneous in trees, presumably caused by the aspiration of air bubbles through conduit walls. Threshold xylem pressures for cavitation vary greatly between species, in concordance with the great functional and ecological diversity of trees. To cite this article: H. Cochard, C. R. Physique 7 (2006).