Pigeons were tested in a successive same-different (S/D) discrimination procedure to examine the short-term memory for individual items in sequences of different or identical pictures. Item-by-item analyses of pecking behavior within single trials revealed this S/D discrimination emerged at the earliest possible point in the sequence--the presentation of the second item. Further, by comparing peck rates at points where different types of sequences diverged (e.g. ABA versus ABC), we determined that the pigeons remembered the first item for at least 4-8s and across one to two intervening items. These results indicate that this S/D discrimination was controlled by relational comparisons of pictorial content across memories of specific items, rather than the detection of low-level perceptual "transients" between items. A second experiment supported this conclusion by showing increased discrimination with longer first item viewing times, consistent with encoding of details about individual pictures. These findings further support a qualitative similarity among birds and primates in possessing a general capacity to judge certain types of stimulus relations, such as stimulus identity and difference. Implications for the temporal continuity of experience in animals are also considered.