Alopecurus myosuroides is one of the most important grass weeds on arable land in Germany. For many years, it has developed herbicide-resistant populations. ACCase- and ALS-inhibiting herbicides, in particular, have lost significant efficacy. Two different mechanisms are commonly known to confer resistance to herbicides: target-site resistance (TSR) and non-target-site resistance (NTSR). In Germany, only approximately 5 to 10% of the herbicide-resistant black-grass populations have been reported to show TSR so far. To quantify the status and the distribution of TSR in Germany, 653 black-grass samples were collected between 2004 and 2012 all over the country. The samples were tested by bioassays and genetic analysis. Averaged over all tested samples, ACCase-TSR increased from 5.0% in 2004 to 54.3% in 2011. In 2012, the proportion of ACCase-TSR was lower than the previous years (38.5%). Similarly, ALS-TSR rose from 0.8% in 2007 to 13.9% in 2012. Regions with a high density of resistant black-grass samples were identified in the north and south of Germany. The data suggest a rapidly working selection of herbicide-resistant populations which may be due to current agricultural practices and the increasing use of herbicides acting on a single site.