A total number of 165 fungi representing 51 genera have been isolated from 22 soil samples collected at various stages of cultivation and crop growth from paddy fields of Orissa. Many rare fungi as well as two gymnoascomycetous fungi new to science have been isolated. Certain of the fungi were found to be ecologically mature and dominant in the paddy fields and were present during all the stages of cultivation or growth of paddy plants. Not much difference in the species of fungi was observed to exist between Cuttack and Sambalpur plots, between fertile and less fertile plots but a definite difference was found between the fungal flora of “dalua” and main crop seasons. Among the four media used, rose bengal streptomycin agar was found to be the best isolation medium.