Micropropagation of 21 species of Restionaceae and the closely related monotypic families Anarthriaceae and Ecdeiocoleaceae is discussed. Multiplication rates ranged from 2-fold to 14-fold each 4–6 week subculture passage, with most species averaging 3–5-fold. The majority of taxa preferred half-strength Murashige and Skoog basal media with 1 μM benzyladenine, with certain species requiring other specific treatments (e.g. Woody Plant medium). Approximately half of the species produced roots successfully (i.e. >50%) in vitro on half-strength MS with no growth regulators (or no auxins), or initiated roots after transfer to soil; other species required a longer (6–7 week) incubation on quarter-strength MS medium for initiation to occur. This paper describes the first successful micropropagation of these taxa with application for horticultural development of this important southern-hemisphere family.