Generation of Induced Pluripotent Stem Cells in Rabbits
Generation of Induced Pluripotent Stem Cells in Rabbits
We describe a procedure for generating induced pluripotent stem cell lines in rabbits, using retroviral vectors expressing Oct4, Sox2, Klf4, and c-Myc of human origin to reprogram rabbit fibroblasts prepared from an ear skin biopsy. We also provide detailed procedures for characterizing the resulting iPSC lines, including the analysis of pluripotency marker expression by RT-qPCR, immunolabeling, and fluorescent-associated cell sorting, the evaluation of pluripotency by teratoma production and genetic stability by karyotyping.
induced pluripotent stem cells, [SDV]Life Sciences [q-bio], Sox2, Genetic Vectors, Induced Pluripotent Stem Cells, rabbit, Cell Culture Techniques, Kruppel-Like Transcription Factors, Gene Expression, Oct4, Mice, SCID, [INFO] Computer Science [cs], Morula, Proto-Oncogene Proteins c-myc, Kruppel-Like Factor 4, Animals, Humans, [INFO]Computer Science [cs], Cellular Reprogramming Techniques, Ear, External, Cells, Cultured, Chimera, retroviral vectors, reprogramming, Cell Differentiation, Fibroblasts, pluripotency genes, Cellular Reprogramming, skin fibroblasts, Klf4, [SDV] Life Sciences [q-bio], c-Myc, Karyotyping, Octamer Transcription Factor-3
induced pluripotent stem cells, [SDV]Life Sciences [q-bio], Sox2, Genetic Vectors, Induced Pluripotent Stem Cells, rabbit, Cell Culture Techniques, Kruppel-Like Transcription Factors, Gene Expression, Oct4, Mice, SCID, [INFO] Computer Science [cs], Morula, Proto-Oncogene Proteins c-myc, Kruppel-Like Factor 4, Animals, Humans, [INFO]Computer Science [cs], Cellular Reprogramming Techniques, Ear, External, Cells, Cultured, Chimera, retroviral vectors, reprogramming, Cell Differentiation, Fibroblasts, pluripotency genes, Cellular Reprogramming, skin fibroblasts, Klf4, [SDV] Life Sciences [q-bio], c-Myc, Karyotyping, Octamer Transcription Factor-3
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