An efficient method to stably insert DNA into a genome is absolutely essential for a modern model system. In general, grasses are notoriously difficult to transform. Fortunately, Brachypodium distachyon has proven to be highly amenable to tissue culture and transformation. Indeed, current transformation methods make B. distachyon one of the easiest and most efficiently transformed grasses. This amenability to transformation is one of the key features that led to the widespread adoption of B. distachyon as a model for the grasses. In addition to being used for a host of functional experiments aimed at specific genes, high-efficiency transformation has enabled the creation of a large collection of sequence indexed insertional mutants. This T-DNA collection is a valuable experimental resource that has been utilized by hundreds of researchers around the world.