AbstractHessian fly (HF; Mayetiola destructor Say) causes severe damage to wheat (Triticum aestivum L.) worldwide. Several resistance genes have been identified in wheat and wild relatives; however, HF populations are under strong selection pressure and evolve rapidly to overcome resistance. To ensure the availability of resistance sources, HF‐resistant germplasm KS18WGRC65 (TA5110, Reg. no. GP‐1042, PI 688251) was developed by Wheat Genetics Resource Center at Kansas State University as a breeding stock that carries resistance gene H26 from Aegilops tauschii Coss. KS18WGRC65 is a cytogenetically stable, homozygous, BC3F3:6 line derived from the cross between Ae. tauschii accession KU2147 and hard red winter wheat recurrent parent ‘Overley’. KS18WGRC65 exhibited no penalty for yield or other agronomic characters, making it a suitable source of HF resistance for wheat breeding.