Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization
Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization
The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
[SDV]Life Sciences [q-bio], Nude, Messenger, Oligonucleotides, Mice, MESH: Oligonucleotides, MESH: Reverse Transcriptase Polymerase Chain Reaction, Bone sarcoma, MESH: Animals, In Situ Hybridization, Tumor, Chromogenic in situ hybridization, MESH: Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, MESH: DNA, MESH: Bone and Bones, Primers, [SDV] Life Sciences [q-bio], MESH: Xenograft Model Antitumor Assays, Histology, mRNA, Mice, Nude, Real-Time Polymerase Chain Reaction, Bone and Bones, MESH: Gene Expression Profiling, MESH: In Situ Hybridization, MESH: RNA, Cell Line, Tumor, Animals, Humans, RNA, Messenger, Bone, MESH: Mice, miRNA, MESH: Humans, LNA probes, Histocytological Preparation Techniques, Gene Expression Profiling, MESH: Digoxigenin, RT-qPCR, DNA, Xenograft Model Antitumor Assays, MESH: Cell Line, MESH: Histocytological Preparation Techniques, MicroRNAs, Gene expression, MESH: MicroRNAs, Digoxigenin
[SDV]Life Sciences [q-bio], Nude, Messenger, Oligonucleotides, Mice, MESH: Oligonucleotides, MESH: Reverse Transcriptase Polymerase Chain Reaction, Bone sarcoma, MESH: Animals, In Situ Hybridization, Tumor, Chromogenic in situ hybridization, MESH: Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, MESH: DNA, MESH: Bone and Bones, Primers, [SDV] Life Sciences [q-bio], MESH: Xenograft Model Antitumor Assays, Histology, mRNA, Mice, Nude, Real-Time Polymerase Chain Reaction, Bone and Bones, MESH: Gene Expression Profiling, MESH: In Situ Hybridization, MESH: RNA, Cell Line, Tumor, Animals, Humans, RNA, Messenger, Bone, MESH: Mice, miRNA, MESH: Humans, LNA probes, Histocytological Preparation Techniques, Gene Expression Profiling, MESH: Digoxigenin, RT-qPCR, DNA, Xenograft Model Antitumor Assays, MESH: Cell Line, MESH: Histocytological Preparation Techniques, MicroRNAs, Gene expression, MESH: MicroRNAs, Digoxigenin
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).3 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!