Data from: Concerted evolution reveals co-adapted amino acid substitutions in Na+K+ ATPase of frogs that prey on toxic toads

Authors: Mohammadi, Shabnam; Yang, Lu; Harpak, Arbel; Herrera-Álvarez, Santiago; Rodríguez-Ordoñez, María del Pilar; Peng, Julie; Zhang, Karen; +4 Authors

doi: 10.5281/zenodo.4677690 , 10.5281/zenodo.4677691

Data from: Concerted evolution reveals co-adapted amino acid substitutions in Na+K+ ATPase of frogs that prey on toxic toads

- Summary
- Metrics

Abstract

Gene duplication is an important source of evolutionary innovation, but the functional distinction between duplicates can be opposed by ongoing gene conversion between them. Here we document a tandem duplication of Na+,K+-ATPase subunit α1 (ATP1A1) sharedby frogs in the genus Leptodactylus,a group of species that feeds on toxic toads. One ATP1A1 paralog evolved resistance to toad toxins while the other paralog retained ancestral susceptibility. Frequent non-allelic gene conversion homogenized most of the paralog sequences,yet the two Leptodactylusparalogs are distinguished by 12 amino acid substitutions that were maintained by strong selection that counteracted gene conversion. Protein-engineering experiments show that two substitutions substantially increase toxin resistance, whereas the 10 additional substitutions mitigate deleterious pleiotropic effects on enzyme function. Our results highlight how neofunctionalized gene duplicates can help pinpoint functional substitutions and their interactions with the genetic backgrounds on which they arise.

"NKA Enzyme Assays Raw Data" - raw plate reader data and downstream calculations for enzyme assays used in this study "Mohammadi_ATP1A1_alignment" - alignment of ATP1A1 gene sequences used to generate phylogenetic trees in this study "NKA_Enzyme_Assays_Raw_Data_Readme" - ReadMe file for the enzyme assay raw data Funding provided by: National Institutes of HealthCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000002Award Number: R01- GM115523Funding provided by: National Institutes of HealthCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000002Award Number: R01- HL087216Funding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: OIA-1736249Funding provided by: Deutsche ForschungsgemeinschaftCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659Award Number: DO527/10-1Funding provided by: Simons FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000893Award Number: 633313

Related Organizations

The University of Texas at Austin
United States
King’s University
United States
College of New Jersey
United States
Universität Hamburg
Germany
University of Nebraska System
United States

View all View all

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	1
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average

Usage byUsageCounts

visibility	views	2
download	downloads	1

2
views
1
downloads
Powered by

Found an issue? Give us feedback

visibility

download

1

Average

2

1