This guide is available to researchers submitting an ANR project, or those considering it. It aims to help project coordinators plan ahead to comply with open science requirements and guidelines, as it can sometimes be too late to start reflecting on this once a project has been awarded funding. It can also be used to support project coordinators throughout their projects’ lifecycles, providing answers to questions that may crop up along the way. Used at a project’s planning stage, it aims to improve feasibility in projects that are submitted and awarded funding, to facilitate collaboration between partners in the field of open science, to help hone a water-tight, ambitious open science strategy, to plan ahead for risk management, and to budget (data storage and any potential APCs for example). Finally, it is a time-saving tool, in that it raises a certain number of questions as early as possible in the project’s lifecycle, rather than as the project progresses. This guide is an updated version of: Féret, Romain, Bracco, Laetitia, Cheviron, Stéphanie, Lehoux, Elise, Arènes, Cécile, & Li, Ling. (2020). Improving your ANR project thanks to Open Science (Version 2). Zenodo. https://zenodo.org/record/3769971