In the present work we seek to undertake a semiotic analysis in two episodes of Organic Chemistry class in Higher Education in order to understand semiosis as signs in action in that teaching context. Scientific knowledge is strongly linked to a type of language that uses a variety of signs that act as mediators of language. Semiotics, known as the General Theory of signs, is the science that studies signs and their semiosis - significant processes. The research material was obtained from the follow-up of an Organic Chemistry I discipline, in a public university, in 2016, through the audiovisual record. From the recorded records, the data was organized using the event map tool and later in episodes of analysis. For the present study, we delimited two episodes on conformational analysis of cyclic compounds. We characterize as a sign in these episodes a hybrid that considers both the teacher and the student as part of it. This working hypothesis is based on the admission that the material tool and the graphic tool act as extensions of humans. From the semiotic continuum, it was possible to perceive the complexity of the representative process and how the human hybrid, speech, gestures and material tool formed a sign for that moment, creating conditions for interpretive chains, semiosis of the concept under discussion. Thus, with this analysis, we placed a possibility of traversing the semiotic continuum covering the first, the second and the third, in two episodes of the classroom.