Probiotics are beneficial microorganisms that are used as feed supplements whose activity in the host's digestive tract produces organic acids so that the intestinal microflora is always in a balanced state and nutrient absorption can be optimal. This study aims to examine the effect of probiotic (Pro-B10) supplementation in the diet on egg characteristics and performance of laying hens. A total of 120 hens aged 60 weeks were randomly divided into 4 treatment groups, namely: the group of laying hens fed a diet supplemented with 0.5% Pro-B10 (P1); 1% Pro-B10 (P2); and 1.5% Pro-B10 (P3). The control group of chickens was fed without Pro-B10 (P0). Each treatment with 5 repetitions with 30 hens per repetition. The results showed that the hens in the P3 group had the highest egg production and feed efficiency (P<0.05) compared to the P0, P1 and P2 hen groups. Pro-B10 supplementation in the diet had a significant (P<0.05) effect on egg characteristics.It can be concluded that supplementation of 1.5% probiotic Pro-B10 in feed for Lohmann Brown laying hens from 60-68 weeks of age, can increase egg production and feed efficiency, as well as increase the weight and color of eggshells which are preferred by consumers.