Although studies of the longhorn beetle on the territory of today's Medobory Nature Reserve began as early as 1864, no complete list of species has been published. Here we present the most comprehensive list of Cerambycidae, based on data from our long-term study (1999-2016). As a result, we identified 86 species of longhorn beetles in the Medobory nature reserve, representing 46 genera, 24 tribes and 6 subfamilies. In the regional dimension, the longhorn beetle diversity of the reserve is 59.7% and 85.2% of the species richness of the Western Podillya macroregion and the Medobory ecoregion, respectively. We found that grasslands are habitat for 22.1% of the longhorn beetle fauna, which is crucial for maintaining species diversity in the reserve.