As digitalisation takes root and customer expectations shift, the logistics industry is entering an era of unparalleled transformation. The new logistics entrants are also shaking up the sector, whether start-ups or industrial customers and suppliers. The Malaysian logistics companies have encountered difficulties, especially as the export and import trade keeps increasing. However, development has still failed to track strong trade growth. Thus, this research aims to investigate the factors affecting and influencing the organizational performance of new start-up logistics enterprises in Malaysia. Therefore, quantitative research investigates the direct and indirect relationship between strategic infrastructure development, government support policy, organizational culture, human asset specificity, and organizational performance. The results of this research show a total of seven hypotheses that are statistically and significantly supported, and only one of the hypotheses is not supported in this study.