The conceptual assumption of the expression of human feelings in English, Uzbek, and Karakalpak languages is important because it highlights the cultural and linguistic differences in how emotions are expressed and understood. This knowledge can be particularly useful for individuals who are learning or working with these languages and cultures, as it can help them better understand and communicate with others. Additionally, understanding the nuances of emotional expression in different languages can promote empathy and cross-cultural understanding, which are important for building relationships and promoting positive interactions between people from different backgrounds. That's why, the article aims to present the comparison of conceptual assumption of the expression of human feelings in English, Uzbek, and Karakalpak languages.