This research aimed to develop a scale to measure pre-service teachers' awareness of web 2.0 tools and examine it based on department and grade level. The study included pre-service teachers from a state university across different grades. A scale was developed with 431 participants from the mathematics and science education department, and 125 participants were then assessed using the scale. Data analysis was performed using exploratory factor analysis with IBM SPSS 26. The resulting ‘pre-service teacher awareness scale for web 2.0 tools’ consisted of 18 items and two factors on a 3-point Likert scale, showing good internal consistency (Cronbach's alpha = 0.86). The scale was administered to pre-service teachers in various teaching departments. The study revealed that pre-service physics teachers had the highest average awareness (M = 25.96) among the departments, and 1st-grade pre-service teachers had significantly higher awareness (M = 24.31) compared to other grade levels. This research provides a reliable scale to assess pre-service teachers' awareness of web 2.0 tools and highlights the need for tailored interventions and support in different educational contexts.