MOLECULAR PROBES FOR DISCRIMINATION FROM AMANITA PONDEROSA The use of ITS and LSU primers specific for Amanita pseudovalens comb. nov., stat. nov. on the Ode01-Ode16 samples indicated that all DNA extracts from the Luzianes A and B collections (Ode1-8 and Ode10-12) and from the Portalegre collection (Ode16) were A. pseudovalens comb. nov., stat. nov. (Fig. 6). All extracts that did not amplify with these primers amplified with the positive control primer sets (not shown). Similar results were obtained with some of the herbarium material (Appendix 2).

Published as part of Arraiano-Castilho, Ricardo, Silva, Ana Cristina, Vila-Viçosa, Carlos, Castro, Mário Rui, Morgado, Luís Neves & Oliveira, Paulo, 2022, The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E. - J. Gilbert) Bertault and the importance of taxon-specific PCR primers for identification, pp. 139-157 in Cryptogamie, Mycologie 20 (6) on page 146, DOI: 10.5252/cryptogamie-mycologie2022v43a6, http://zenodo.org/record/7829510