The Corn (Zea mays L) is one of the most important cereals, moit used by the world population. The study of the genetic polymorphism of local corn collections is an important component for enrichment and use in breeding Based on this, it was carried out the evaluation of DNA polymorphism of 16 constant forms and 25 corn hybrids from the National Genebank of Azerbaijan by PCR analysis using 5 ISSR markers, It was identified a high level of polymorphism both in hybrids and n constant forms, averaging 93.24%. It was revealed 8 groups of genotypes by the cluster analysis of the values of Ney genetic distances. Based on the value of genetic diversity index and intormation polymorphism content PIC, the diseriminatory ability of the marker system was evaluated, the most eftective of which was demonstrated by the primer UBC-827 with sequence (AC),G