Hepatoprotective agents are those compounds, which mitigate the liver injury caused by hepatotoxic agents. Hepatoprotective effect of plant drug & herbal formulation are studied against chemicals & drug induced hepatotoxicity in rat & mice as they virtually miniric any form of naturally occurring liver diseaseLiver disorders are considered as one among the most serious ailments. They are mainly caused by toxic chemicals ( carbon tetrachloride), drugs (thiacetamide, paracetamol, antivirals, antibiotics). The outcome of the result of Methanol extract of Citrus limetta on CCl4 induced hepatotoxicity in rats. The effect of administration of CCl4 to rats caused severe liver damages as there was a significance increase in the levels of SGPT,SGOT,ALP,ACP,LDH, total bilirubin where as s significant decrease in the level of total protein was observed which may be due to the acute hepatocellular damage and biliary obstruction.The present results provide strong evidence that the formulation inhibits hepatotoxicity induced by carbon tetrachloride. Pharmacological studies of Citrus limetta plant are at the preliminary level requiring further stidies to delineate the mechanisms of actions. Literature review revealed that Citrus limetta possesses all activities of citrus have also been reported. There are indication that this plant also possesses Hepatoprotective Activity but till date there is no scientific evidence in support of this. Hepatoprotective is not reported till date. So it has been thought worthwhile to study Hepatoprotective of Citrus limetta. Hepatoprotective agents is underway, the world health organization has recommended the development of herbal medicine in this concern. Although alternative treatment are increase being to alleviate effective disorder, the evidence to recommend the use of herbal medicine in the treatment of hepatoprotective, because herbal medicine are widely used and often by the general public, more clinical safety and efficacy.