This document is intended to help ecologists get started using BIIGLE to annotate their image and video data. The manual was originally written to support new members of the Deep-Sea Conservation Research Unit as well as undergraduate students at the University of Plymouth. It is a collection of information that we have found helpful to document in our experience of setting up and using BIIGLE. We intend for this to be a living document that others can contribute to. The manual is hosted on GitHub in our BIIGLE-resources repository. If you see something that you feel could be made clearer or needs changing, please let us know by flagging an issue on GitHub. This can be done by going to our BIIGLE-resources repository, clicking ‘Issues’ (top left), and then logging a ‘New issue’ (green button on the right). This will allow us to update and improve this resource as we intend to do so annually.