This record hosts data generated by the 1000 Soils Pilot. Data will be updated as more become available. Please see the most recent data upload for current data. Our estimated timeline is as follows: Early-to-mid December: Geochemistry, texture, respiration, enzyme activities, and FTICR-MS organic matter chemistry Late February: Microbial biomass C and N; TOC/TDN of water-extractable OM; X-ray computed tomography Mid-April: Metagenomes July: LC-MS organic matter chemistry September: Soil hydraulic properties (specific samples may be available upon request) 1000S_Dataset_Biogeochemistry_2022-12-01.csv contains all available biogeochemical data and will be updated as more data become available. 1000S_Dataset_Biomass_Tomography_WEOM_2023_03_01.xlsx contains microbial biomass C and N; TOC/TDN of water-extractable OM; and X-ray computed tomography. icr.reps.combined_v1.csv contains FTICR-MS data processed by CoreMS. These data are merged by formula across instrument runs to enable cross-sample comparisons. Technical replicates are merged by retaining peaks present in 2 out of 3 replicates. 1000Soils_Metadata_Site_Mastersheet_v1.csv contains site information. Please contact Qian Zhao (qian.zhao@pnnl.gov) or Emily Graham (emily.graham@pnnl.gov) with questions. The following file is available upon request: icr_by_mass_for_single_sample_analysis_only.csv contains FTICR-MS data processed by CoreMS and is intended for usage in the calculation of biochemical transformations within samples only. These data are not acceptable for cross-sample comparison of masses because they are from multiple instrument runs.