FIGURE 2. Phylogenetic relationships among Speckled Dace populations in this study, modified from Su et al (2022). The molecular phylogeny was generated by an ML coalescent model in SVDQuartets with 100 bootstraps, using RAD sequencing data. Numbers on each lineage refer to the percentage of bootstrap runs (out of 100) that produced the same result. Details can be found in Su et al. (2022). The lineage names refer to major drainages from which our Speckled Dace samples were collected from California, as well as from major basins outside of California. We did not examine the latter findings in detail; they probably represent multiple undescribed species/subspecies. The capital letters refer to lineages identified as species in this paper. A. Desert Speckled Dace (R. nevadensis), B. Western Speckled Dace (R. klamathensis), C. Santa Ana Speckled Dace (R. gabrielino). Tui Chub (Siphatales bicolor) and Relict Dace (Relictus solitarius) were outgroups used to root the phylogeny because they are both western cyprinids distantly related to Rhinichthys (Schönhuth et al. 2012).

Published as part of Moyle, Peter B., Buckmaster, Nicholas & Su, Yingxin, 2023, Taxonomy of the Speckled Dace Species Complex (Cypriniformes: Leuciscidae, Rhinichthys) in California, USA, pp. 501-539 in Zootaxa 5249 (5) on page 512, DOI: 10.11646/zootaxa.5249.5.1, http://zenodo.org/record/7701357