A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.132952.2 This report presents a guide to selecting high-quality commercial antibodies against hVPS35 (Vacuolar protein sorting-associated protein 35) by immunoblot (Western blot), immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs iin knockout cell lines and isogenic parental controls. This work was supported in part by the Michael J. Fox Foundation for Parkinson's research.