Human beings rely heavily on medicinal plants to maintain their health. The pharmacological assessment of numerous plants utilized in Indian traditional medicine is becoming increasingly popular. The plant Cymbopogon citratus, commonly known as West Indian lemon grass or simply lemon grass,is a tropical plant native to Maritime Southeast Asia and introduced to many tropical regions Cymbopogon citratus is part of the grass family, Poaceae. In folk medicine, it has been used as antibacterial, antifungal, anti-inflammatory, anticancer, analgesic, antiseptic and antinociceptive and antioxidant agents Due to its capacity to function as an antibiotic and antiseptic, it can be used to treat ringworm and athlete's foot disease. Lemongrass has a strong antimicrobial effect against methicillin-resistant Staphylococcus aureus (MRSA). It can help with colitis, indigestion, and gastro-enteritis. It aids in the relief of headache, bodily discomfort, nervous weariness, and other stress-related symptoms It has been used to treat gastrointestinal disorders. In cases of fever, a decoction of lemongrass leaves is used as a diaphoretic lemongrass revitalizes the body and improves overall wellness. This study deals with Extraction, Phytochemical screening and in vitro antioxidant activity of Cymbopogon citratus. The leaves plant was collected and subjected to extraction in hydroalcoholic solvent. The phytochemical screening & antioxidant activity was also assessed. The results showed that the percentage yields were found to be (7.21% w/w of crude drug) of Hydroalcoholic extract Cymbopogon citratus leaves. Results of Phytochemical test showed the presence of Carbohydrates, Flavonoids, Proteins & Amino acids, Diterpenes and Saponins. Phenols and Alkaloid were found to absent in extract Cymbopogon citratus. The results of phytochemical revels that the all polar and Methanolic and aqueous soluble compound was found to be present in Cymbopogon citratus extract. Antioxidant activity of the samples was calculated through DPPH assay. % inhibition was calculated as an indicative of antioxidant potency. The percentage inhibition for ascorbic acid was found to be 14.23% while for Hydroalcoholic extract Cymbopogon citratus leaves the IC50 value was found to be 88.72%. Results point towards fact that Cymbopogon citratus exhibit potent antioxidant activity. Keywords: Cymbopogon citratus, Antioxidant activity, Herbal medicines, IC50