Abstract Bats are an essential part of many ecosystems. However, their natural habitats are in a steady state of decline in many areas. In an attempt to mitigate habitat loss and supplement roosting sites, artificial roosts known as bat houses have been installed in targeted areas. Many factors must be considered when creating an ideal bat house, such as colour, size, installation location, the material of construction, and overall design. Several studies have identified that weather conditions can also influence occupancy rates in bat houses. Staff, volunteers, and student interns at Beaverhill Bird Observatory (BBO) have been monitoring and studying bat house occupancy in the Beaverhill Natural Area (BNA) since 2016. Several peer reviewed studies have noted the importance of microclimatic and weather conditions as an influence on bat house occupancy. However, the specifics of what aspects of microclimatic and weather conditions were never identified. In this study, weather conditions, including average temperature during sampling and humidity were documented and compared to preferred bat roosting locations. Additionally, the proximity to water relating to bat house locations was considered part of the scope of this study. Interpretation of the data collected suggested that there was a low correlation between bat house occupancy, temperature, and humidity. When reviewing data related to bat house locations and proximity to water, the study revealed a strong correlation to bat house occupancy (R² = 62%).