The metagenomics dataset was collected from the persistent microbial bloom from the chemocline (oxic-anoxic boundary) of Lake Cadagno. The location of the chemocline was between 13-15.5 m in depth on the day of sampling (29 August 2017). There are a total of 4 metagenomics samples collected between 13-15.5m depth labeled as 13m, 15mm, 15mw (without mesh), and 15_5m. If you use this dataset, please cite both the article and Zenodo source 1) Saini, J.S., Manni, M., Hassler, C. et al. Genomic insights into the coupling of a Chlorella-like microeukaryote and sulfur bacteria in the chemocline of permanently stratified Lake Cadagno. ISME J 17, 903–915 (2023). https://doi.org/10.1038/s41396-023-01396-y 2) Jaspreet Singh Saini, Mosè Manni, Christel Hassler, Rachel Cable, Melissa B. Duhaime, & Evgeny M. Zdobnov. (2023). Metagenomics Dataset From Chemocline of Lake Cadagno [Data set]. Zenodo. https://doi.org/10.5281/zenodo.7505505