Healthcare workers are the strongest pillars in providing quality healthcare services and play a vital role in maintaining a healthy state and achieving health-related goals such as health promotion, disease prevention, health restoration, institutional care, and rehabilitation services. However, these healthcare workers remain an invisible workforce in the healthcare industry. This study investigates the effect of job-related stress on the job satisfaction of HCWs working in hospitals. Work stress is one of the most important health risks in the workplace for employees, and job satisfaction has been considered a critical factor in providing high-quality services and outstanding performance in hospitals. This study presents a field survey. Drawing on a sample of 357 healthcare workers working in Najran hospitals, we examined the degree to which stressors such as workload, conflict, career development, interpersonal relationships, and access to information influence aspects of job satisfaction such as the physical environment, job opportunities, and style. Management, job enrichment, rewards and job security. The results showed that conflict, heavy workload, and lack of job autonomy were negatively associated with all dimensions of job satisfaction. Conclusion: Healthcare administrators, especially HCWs administrators, should consider the factors that contribute to job dissatisfaction, work stress, and try to eliminate them by designing and implementing helpful policies and practices. One aspect that must also be addressed is an increase in continuing education programs for HCWs, especially programs that deal with stress and conflict management, strengthening coping mechanisms, enhancing personal skills and achievements, and updating knowledge. Keywords: Health-care worker, Work-Related Stress, job satisfaction, Saudi Arabia