The in vivo technique of getting mature roe from the parental sturgeon fish has been introduced in Khilli sturgeon hatchery for the first time in sturgeon-breeding practice of Azerbaijan Republic. The mature roe is got from the parental fish by conducting Caesarean and oviduct sections. The parental sturgeon fish from is being kept in the Khilli Sturgeon Hatchery for the purpose of their domestication. In the Khilli Sturgeon Hatchery the repair-maternal stock of sturgeon fish is formed by applying the juveniles of artificial generation. For the purpose of biodiversity protection, the application of parental sturgeon fish breeders of different biological groups as well as extinct sturgeon fish (Kura-river ship) for maternal stock formation is considered to be important.