Plumbago indica (Plumbaginaceae) named “agnichita” belonging to the family Plumbaginaceae. The family is sometimes referred to as the leadwort family or the plumbago family. Most species in this family are perennial herbaceous plants, but a few grow as shrubs. In present investigation aim to evaluate the antidiabetic effect of roots extract of Plumbago indica. In our study, there was decrease in blood glucose level was found in all treatment groups during study. Progressive decrease in blood glucose level was found in all treatment groups during study. At the end of experiment Glibenclamide 500 mcg/kgp.o., Plumbago indica 200 and 300 mg/kg/p.o. (110.00 ± 6.50; 116.00 ± 6.00 and 118.00 ± 5.50) treated group blood glucose level was decrease significantly (p<0.05) at 21st days, respectively. Impaired carbohydrate metabolism and developing insulin resistance is the main metabolic disorder in non- insulin dependent diabetes mellitus leading to hyperglycemia. Altered digestion and absorption of dietary carbohydrate, depletion of glycogen storage, increased gluconeogenesis, β-cell dysfunction, insulin resistance of peripheral tissue and defect in insulin signaling pathways are important causes of hyperglycemia. Key words: Plumbago indica, Extraction, Phytochemical, Antidiabetic activity,