In this paper are presented values of biochemical parameters of blood of 11 Gatačko breed cows in different reproductive or production stages and the results of food analysis. Hyperproteinemy and hyperglobulinemy were found in all, and hyperalbuminemy was found in 8 tested animals (72.72%). The concentration of urea below the lower physiological limit was found in 8 animals (72.72%) (mean value 6,06±0,54 mmol/L). The cholesterol concentration in 9 animals (81.81%) was above the upper physiological limit (mean value 4,16±0,42 mmol/L). Hyperbilirubinemy was found in all tested animals (mean value 25,55±2,62 mmol/L). Calcium blood level was below the lower physiological limit in 4 animals (36.36%). Phosphorous blood level was below the lower physiological limit in one animal (9.09%), and above the upper physiological limit in remaining 10 (91.91%). ALT activity was elevated in 10 animals (91.91%) (mean value 71,27±4,64 U/L). The average activity of AST was within physiological values, and the increase was found in 5 animals (45.45%). Analysis of the meal showed that meal was insufficient for animals in crude protein and energy content.