ABSTRACT The aim of this work is to study the effect of a composite endomycorrhizal inoculum on growth of Eucalyptus plants (E. gomphocephala) in the nurseries conditions. Analysis of the results, four months after inoculation, revealed that all roots were mycorrhizal and different structures characterizing arbuscular endomycorrhizal fungi were observed. The mycorrhizal frequency and intensity of Eucalyptus roots are 90 and 25% respectively. This roots endomycorrhization was accompanied by a significant improvement on growth parameters of mycorrhized Eucalyptus plants compared to the control, these are respectively, plant height 90.31/75.13 cm, root height 57/30.37cm, leaves number 106.75/79.12 leaves per plant, stem diameter 0,61/0.32 cm, average number of branch 11.62/6.5 and the fresh weight of the root and vegetative part of mycorrhizal plants are more important compared to the control. The fresh weight gain was, on average, 17.54g in the stems and of 24.37g in the roots. The number of spores in the rhizosphere of the inoculated plants is about 180 spores/100g of soil; the identification of these spores has allowed to note the presence of 33 species, divided into 8 genera: Glomus, Acaulospora, Scutellospora, Pacispora, Gigaspora, Redeckera, Rhizophagus and Entrophospora. The importance of Eucalyptus plants endomycorrhization compared to the ectomycorrhization was also discussed in this study. Keywords: Eucalyptus , Endomycorrhizae, AMF, mycorrhization REFERENCES Adouane M., 2011. Diversité des macromycètes et des mycorhizes du chêne-liège dans deux stations du Nord-Est Algérien: une subéraie naturelle et une subéraie envahie par Acacia decurrens (Willd). Mémoire de Magistère,Faculté des Sciences, Universite Badji Mokhtar / Annaba, Algérie, 183p. Adouane M., Beddiara A., 2011. « La gestions des subéraies et la qualité du liège ». Deuxièmes Rencontres Méditerranéennes Chercheurs-Gestionnaires-Industriels Jijel, 17-19. Abourouh M., 1994. Les Ectomycorhizes du Cèdre de l’Atlas : état des connaissances et perspectives. In : Le Cèdre de l’Atlas, Cedrus atlantica (Manetti). Silva Mediterranea / O. M’Hirit, A. Samih, M. Malagnoux Eds. Actes du Séminaire international sur le Cèdre de l’Atlas, Ifrane (Maroc), 7-11 juin 1993. Annales de la Recherche forestière au Maroc, 27 (spécial), 1 : 337-348. Abourouh M., 2000. Mycorhizes et Mycorhization Des Principales Essences Forestieres Du Maroc. Thèse de Doctorat D'Etat es-Sciences. Université Mohamed V, Faculté des sciences, Rabat, Maroc. Aldwell FEB, Hall IR., 1987. A review of serological techniques for the identification of mycorrhizal fungi. In: Sylvia DM, Hung LL, Graham JH (eds) Mycorrhizae in the next decade. Practical applications and research priorities. Proceedings of the 7th North American Conference on Mycorrhizae, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Fla., pp 305–307 Aouadj R., Essgaouri A., Abourouh M., 1997. Croissance et assimilation des nitrates chez le champignon ectomycorhizien Pisolithus tinctorius. Revue Marocaine des Sciences Agronomiques et Vétérinaires, 17 (4) : 209- 216. Aouadj R., Essgaouri A., Button B., 2000. Etude de la stabilité et de quelques propriétés de la nitrate réductase du champignon ectomycorhizien Pisolithus tinctorius. Cryptogamie Mycologie, 21 (3): 187–202. Arriagada C., Pacheco.P., Pereira G., Machuca A., Alvear M., and Ocampo J.A., 2009. Effect of arbuscular mycorrhizal fungal inoculation on Eucalyptus globulus seedlings and some soil enzyme activities under application of sewage sludge amendment. Rev. Cienc. Suelo Nutr. / J. Soil. Sci. Plant Nutr., 9(2): 89-101. Asai T., 1934. Uber das Vorkommen und die Bedeutung der Wurzelpilze in den Landpflanzen. Japanese Jour. Bot., 7:107-150. Ashford A.E., Ling Lee M. and Chilvers A., 1975. Polyphosphates in Eucalypt mycorrhizas. A cytochemical demonstration. New Phytol., 74: 447- 453. Bakkali Yakhlef S., Kerdouh B., Mousain D., Ducousso M., Duponnois R., Abourouh M., 2009a. Phylogenetic diversity of Moroccan cork oak woodlands fungi, Biotechnol. Agron. Soc. Environ., 13(4): 521-528. Bakkali Yakhlef S., Mousain D., Duponnois R., Ducousso M., Belkouri A., Kerdouh B., Perrineau M. et Abourouh M., 2009b. Molecular phylogeny of Pisolithus species from Moroccan forests woodlands. Symbiosis, 49(3): 157-162. Bakkali Yakhlef S.E., Abourouh M., Ducousso M., Duponnois R, Delaruelle C., Mousain D., 2011. Intraspecific variability of Pisolithus spp. as a response to changes in soil characteristics in a Moroccan cork oak plantation. Mycology, 2(4): 283–290. Baylis B.T.S., 1971. Endogonaceous mycorrhizas synthesized in Leptospermum (Myrtaceael). New Zeal. Jour. Bot., 9: 293- 296. Belkouri A., Bakkali Yakhlef S., Es-sgaouri A., Aouadj R. et Abourouh M., 2009a. Tolérance au stress hydrique et à la salinité et caractérisation moléculaire des isolats de Pisolithus spp., récoltés sous Eucalyptus au Maroc. Annales de la Recherche Forestière, 40 : 3 – 16. Belkouri A., Bakkali Yakhlef S E., Essgaouri A., Aouadj R. et Abourouh M., 2010. Activité antagoniste et caractérisation moléculaire des isolats de Pisolithus spp., récoltés en forêt de la Mamora (Maroc). IOBC/wprs Bulletin, 57: 97- 101. Belkouri A., 2011. Contribution à l’étude de la biodivérsité des champignons éctomycorhiziens Pisolithus spp. au Maroc.Thèse de Doctorat d’Etat, Université Hassan II, Ain Choc, Faculté des Sciences, Casablanca, Maroc, 174p. Berch S., 1986. Endogonaceae: taxonomy, specificity, fossil record, phylogeny. Front Appl Microbiol 2 : 161-188. Boudarga K . et Dexheimer J., 1988. Étude ultrastructurale des endomycorhizes à vésicules et arbuscules de jeunes plants d'Eucalyptus camaldulensis (Dehnardt) (Myrtacées), Bulletin de la Société Botanique de France. Lettres Botaniques, 135(2) : 111-121 Boudarga K . et Dexheimer J., 1989. Sur la mycorhization contrôlée de semis d’Eucalyptus camaldulensis Dehnardt par Gigaspora margarita Becker & Hal. Annales des sciences forestières, 46 (2) :131-139. Bougher N. L., 1995. Diversity of ectomycorrhizal fungi associated with Eucalyptus in Australia, in Mycorrhizas for plantation forestry in Asia, M. Brundrett, B. Dell, N. Malajczuk, and M. Q. Gong, Eds., Proceedings of the N◦62, pp. 8–15, Australian Centre for International Agricultural Research, Canberra, Australia. Campos D.T.S, da Silva M.C.S, da Luz J.M.R, e Maria. R.J.T., Kasuya. C.M., 2011. Colonização Micorrízica Em Plantios De Eucalipto. Revista Árvore, Viçosa-MG, 35(5) : 965-974. Chen, Y.L., Brundrett, M.C. & Dell, B., 2000. Effects of ectomycorrhizas and vesicular-arbuscular mycorrhizas, alone or in competition, on root colonization and growth of Eucalyptus globules and E. urophylla. New Phytologist, 146: 545-556. Chen Y.L., Liu S., and Dell B., 2007. Mycorrhizal status of Eucalyptus plantations in South China and implications for management. Mycorrhiza, 17: 527–535. Chilvers, G.A., 1968 a. Law-power electron microscopy of the root cap region of Eucalypt mycorrhizas. New Phytol., 67: 663- 665. Chilvers, G.A., 1968 b. Some distinctive types of Eucalyptus mycorrhizas. Aust. J. Bot., 16: 49- 70. Chilvers, G.A., 1972. Host range of some eucalypt mycorrhizal fungi. Australian Journal of Botany, 21: 103-111. Chilvers G.A. et Gust LW. 1982. The development of mycorrhizal populations on potgrown seedlings of Eucalyptus St-johni R.T. Ba k. New Phytol., 90: 677- 699. Chliyeh M., Touati J., Selmaoui K., AminaOuazzani,Touhami, Filali-Maltouf A., El Modafar C., Moukhli A., Benkirane R., and Douira A. . 2014. Bibliographic inventory of the endomycorrhizal species associated with the olive tree (Olea europaea L.). Biolife. 3(1); 228-234 Clark, R.B., et Zeto, S.K., 2000.Mineral acquisition by arbuscular mycorrhizal plants, Journal of Plan Nutrition, 23:867-902 Cooper K.M., 1976. A field survey of mycorrhizas in New Zealand ferns. New Zeal. Jour. Bot., 14: 169- 181. Dalpé Y., 1995. Gigaspora margarita.Fungi Canadenses No. 331. Can.J.Pl. Pathol., 16: 229-230. De La Cruz R.E., Bartolome H.T., AGGANGAN N.S., 1988. Pilot testing of mycorrhizal tablets for pines and Eucalyptus in the Philippines. In : Proceedings UNESCO Regional Workshop on Development and Production of Mycorrhizal Inoculants. Biotech, UPLB College Laguna, Philippines. De Mendonça Bellei M., Garbaye J., and Gil M., 1992. Mycorrhizal succession in young Eucalyptus viminalis plantations in Santa Catarina (southern Brazil). For. Ecol. Manage, 54: 205–13. Derkowska E., Sas Paszt L., Sumorok B., Szwonek E., Głuszek S., 2008. The influence of mycorrhization andorganic mulches on mycorrhizal frequency in apple and strawberry roots. J. Fruit Ornam. Plantes, 16: 227-242. Dominik T., 1956. Synopsis of a new classification of the ectotrophic mycorrhizae established on morphological and anatomical characteristics. Mycopathologia, 11 (4) : 359-367 Ducousso M., Duponnois R., Thoen D., et Prin Y., 2012. Diversity of Ectomycorrhizal Fungi Associated with Eucalyptus in Africa and Madagascar. Hindawi Publishing Corporation International Journal of Forestry Research, Article ID 450715, 10 pages, http://dx.doi.org/10.1155/2012/450715 Ferrer RL. et Herrera RA., 1981. El género Gigaspora Gerdemann et Trappe (Endogonaceae) en Cuba. Rev. Jardín. Bot. Nacional, Habana 1: 43–66. Garbaye J., Delwaulle J.-C., Diangana D., 1988. Growth response of Eucalyptus in the Congo to ectomycorrhizal inoculation. Forest Ecology and Management, 24 : 151-157. Gardes M., Bialet E., Binet E., Brousseau C., Carré F., Charcosset J.Y., Fradet N., Griffith P., Gryta H., Laquerbe M., Martinez C. et Millot S., 2003. Les symbiotes mycorhiziens du peuplier noir (Populus nigra L.) : la spécificité des assemblages fongiques en milieu riverain. Les Actes du BRG, 4 : 453-466. Giachini A., Oliveira V. L., Castellano M. A., et Trappe J. M., 2000. Ectomycorrhizal fungi in Eucalyptus and Pinus plantations in southern Brazil. Mycologia, 92 (1–6): 1166–1177. Govindarajulu M, Pfeffer PE, Jin HR Abubaker J, Douds DD, Allen JW, Bucking H, Lammers PJ, Shachar-Hill Y., 2005. Nitrogen transfer in the arbuscular mycorrhizal symbiosis. Nature, 435: 819-823. Gerdemann, J.W. and Nicolson, T.H., 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc., 46: 235-244 Govindarajulu, M., Pfeffer, P.E., Jin, H., Abubaker, J., Douds, D.D., Allen, J.W., Bücking, H., Lammers, P.J.,and Shachar_Hill, Y., 2005. Nitrogen transfer in the arbuscular mycorrhizal symbiosis. Nature, 435: 819-823 Grand L.F., 1969. A beaded endotrophic mycorrhiza of northern and southern red oak. Mycologia, 51:408- 409. Grove T.S., Malajczuk N., Burgess T., Thomson B.D., HARDY G., 1991. Growth responses of plantation eucalypts to inoculation with selected ectomycorrhizal fungi. In : IUFRO Symposium on Intensive Forestry : The Role of Eucalypts / A.P.G. Schînau Ed. Pretoria : South African Institute of Forestry, pp. 86-93. Grove T.S., Le Tacon F., 1993. Mycorrhiza in plantation forestry. In : Mycorrhiza Synthesis / I.C. Tommerup Ed. Advances in Plant Pathology, 9: 191-227. Khan A.G., 1978. Vesicular arbuscular mycorrhizas in plants colonizing black wastes from bituminous coal mining in the illawarra region of New South Wales. New Phytol., 81: 53 - 63. Kisa M, Sanon A, Thioulouse J, Assigbetse K, Sylla S, Spichiger R,Dieng L, Ber- thelin J, Prin Y, Galiana A, Lepage M, Duponnois R., 2007. Arbuscular mycorrhizal symbiosis can counterbalance the negative influence of the exotic tree species Eucalyptus camaldulensis on the structure and functioning of soil microbial communities in a sahelian soil. FEMS Microbiology Ecology, 62(1):32–44. Köske R.E. and Gemma J.N., 1989. A Modified Procedure for Staining Root to Detect VAM. Mycological Research, 92: 486-505. Lamb D, Erskine P.D., Parrotta J.A., 2005. Restoration of degraded tropical forest landscapes. Science, 310(5754):1628–1632. Lapeyrie FF, Chilvers GA., 1985. An endomycorrhiza-ectomycorrhiza succession associated with enhanced growth of Eucalyptus dumosa seedlings planted in a calcareous soil. New Phytologist, 100: 93-104. Li L., Li T., Zhao Z., 2007. Differences of arbuscular mycorrhizal fungal diversity and community between a cultivated land, an old-field, and a never-cultivated field in a hot and arid ecosystem of southwest China. Mycorrhiza, 17(8): 655-65. Lodge D.J., 1990. Negative associations among VA-mycorrhizal fungi and some ectomycorrhizal fungi inhabiting the same root system. Oikos, 57: 347-356. Lopez Aguillon R., Garbaye J., 1990. Some aspects of a double symbiosis with ectomycorrhizal and VAM fungi. Agricul. Ecosyst., 29: 263-266. Maeda M., 1954. The meaning of mycorrhiza in regard to systematic botany. Kumamoto Sei. Ser. B., 3: 57- 84. Malajczuk N., 1981. Presence of vesicular-arbuscular mycorrhizes in Eucalyptus spp. and Acacia sp. and their absence in Barkia sp. after inoculation with Glomus fasciculatus. New Phytol., 87: 567- 572. Malajczulk N., MOLINA A. et TRAPPE J.M., 1982. Formation in Eucalyptus. 1. Pure culture synthesis, host specificity and mycorrhizal compatibility in Pinus radiata. New Phytol., 91: 467- 482. Malajczulk N., 1984. Formation in Eucalyptus. II. The ultrastructure of compatible and incompatible mycorrhizal fungi and associated roots. New Phytol., 96: 43-53. Marx D.H., Ruehle J.L. et Cordell C.E., 1991. Methods for studying nursery and field response of trees to specific ectomycorrhiza. In : Methods in microbiology, Vol 23, Norris, J.R., Read, D.J. et Varma, A.K. (éds.), Academic Press, London, pp. 383-41 1. Meddich A., Oihabi A., Abbas Y., Bizid I.E., 2000. Rôle des champignons mycorhiziens à arbuscules de zones arides dans la résistance du trèfle (Trifolium alexandrinum L.) au déficit hydrique. Agronomie, 20 : 283–295 Morton J.B., Benny G.L, 1990. Revised classification of arcuscular mycorrhizal fungi (Zygomycetes) : A new order, Glomales, two new busorders, Glomineae and Gigasporineae, with an emendation of Glomzceae. Mycotaxon, 37 : 471-491. Mouaya T., 1989. Contribution à l’étude de la Mycorhization Contrôlée des Plants d’Eucalyptus urophylla issus de semis par quelques souches de Pisolithus arhizus. Mémoire de fin d’étude, Université Marien Ngouabi, Brazzaville, Congo. Mukerji KG., 1996. Taxonomy of endomycorrhizal fungi . In Mukerji KG, Mathur B, Chamola BP, Chitralekha P. (eds.) Advances in Botany, 213-221. APH Publishing Corporation, New Delhi, pp. 211-221. Nouaïm R. et Chaussod R., 1997. Effet de la mycorhization contrôlée sur la croissance de I'arganier (Argania spinosa) après sa transplantation en sol non désinfecté. Al Awamia, 96: 65-76. Nounsi A., Outcoumit A., Selmaoui K., Ouazzani Touhami A., Benkirane R. et Douira A., 2014. Inventaire des champignons ectomycorhiziens du Maroc. Journal of Applied Biosciences, 79:6826 – 6854. Oliveira V.L., Schmidt V.D.B., and Bellei M.M., 1997. Patterns of arbuscular and ecto- mycorrhizal colonization of Eucalyptus dunnii in southern Brazil. Ann. For. Sci., 54: 473–481. Oihabi A., 1991. Étude de l’influence des endomycorhizes à vésicules et arbuscules sur le Bayoud et la nutrition du palmier dattier. Thèse de Doctorat d’État, Univ. Cadi Ayyad, Marrakech, Maroc, 117 p. Oihabi A., Meddich A., 1996. Effet des mycorhizes à arbuscules sur la croissance et la composition minérale du trèfle (Trifolium alexandrinum). Cahiers Agriculture, 5 : 382-386. Outcoumit A., 2011. Contribution à l’étude de la diversité fongique du Maroc et mise en évidence de quelques espèces fongicoles et de l’importance des espèces sécotïdes dans la systématique des Basidiomycètes. Thèse de Doctorat, Université Ibn Tofaïl, Faculté des Sciences, Kénitra, Maroc, 497p. Philips J.M., Hayman D.S., 1970. Improved procedures for clearing rots and staining parasitic and vesicular arbuscular mycorhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc., 5:158-161. Plassard C., Scheromm P., Mousain D., Bousquet N. et Salsac L., 1988. Ectomycorhizes et nutrition minérale - Relations source-puits. Bull. Soc. bot. Fr., 135, Actual. Bot., (1): 109-118. Plassard C., Chalot M., Botton B., Martin F., 1997. Le rôle des Ectomycorhizes dans la nutrition azotée des arbres forestiers. Le fonctionnement des symbioses mycorhiziennes Rev. For. Fr. XLIX - n° sp. : 82-98. Plenchette C., et Strullu DG., 1996. Les mycorhizes, situation et perspectives pour le pépiniériste et l’horticulteur. PHM Revue Horticole, 365: 72-76 Reddell P. et Malajczulk N., 1984. Formation of mycorrhiza by jarrah (Eucalyptus marginata Donn ex Smith) in litter and sail. Aust. J. Bot., 32: 511 - 520. Ruiz-Lozano J.M., Azcon R., Gomez M., 1995. Effect of arbuscular mycorrhizal Glomus species on drought tolerance: physiological and nutritional plant responses, Appl. Environ. Microbiol., 61: 456–460. Schenck N.C., Smith G., 1982. Additional new unreported species of mycorrhizal fungi (Endogonaceae) from Florida. Mycologia, 74: 77-92. Schenck N.C., Perez Y., 1987. Manual for the Identification of VA Mycorrhizal Fungi. Second Edition. International Culture Collection of VA Mycorrhizal Fungi (INVAM), University of Florida, Gainesville, Florida. Sghir F., Touati J., Chliyeh M., Ouazzani Touhami A., Filali-Maltouf A., El Modafar M., Moukhli A., Oukabli A., Benkirane B. an