The purpose of this paper was to examine the relationship between Locus of Control and academic achievement of male and female under B.Ed pupil teachers of Rohtak district. The study was conducted on a sample of 120 male and female of B.Ed pupil teachers, from B.Ed colleges of Rohtak district. Then adaptation of Hasnain and Joshi’s Locus of Control scale was used to assess the locus of control of the pupil teachers. The result indicated that there was no significant positive correlation between external locus of control and academic achievement of the pupil teachers. Futher it was found that male and female of B.Ed pupil teachers having internal Locus of Control help better academic achievement than male and female of B.Ed pupil teachers their external Locus of Control.